13774214275
13774214275
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BsaI
is a Type IIs restriction enzyme that can recognize non-palindromic sequences
and cut outside of the recognition sequence. It is commonly used for Golden
Gate assembly and enzymatic cleavage of plasmids to prepare linear DNA
fragments with poly （A/T/G/C） endings and obtain specific sticky ends.
Recognition
site:
5'-GGTCTC（N）1↓-3'
3'-CCAGAG（N）5↑-5'
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